Part:BBa_K750008

Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

This part contains BBa_K750001 and BBa_K750006. Activated by arabinose, this device will produce protein LuxI and protein LuxR. The LuxI protein synthesizes a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumulates as the cell density increases, and it will bind with the protein luxR at a high concentration. The LuxR-AHL can activate the promoter LuxPR, and finally lead to expression of protein GFP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  Illegal NheI site found at 1069
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 793
  Illegal BamHI site found at 65
  Illegal BamHI site found at 1009
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]